Traditional submerged cell culture techniques have well-known limitations for aerosol exposure studies; for instance, the impact of exposure dilution within the media and the poor solubility/sedimentation of some exposure particles1. Air liquid interface (ALI) cultures are a technique whereby cells are grown on a microporous membrane insert, immersed in a media-containing well.
Acute lung injury (ALI) arises from factors as diverse as endotoxin exposure, chemical assault, acute particulate exposures or mechanical impacts, as seen in ventilator induced lung injury (VILI). ALI and VILI studies push the understanding of both fundamental breathing mechanics and general lung physiology to describe how damage occurs in the hopes of mitigating of damage.
Longitudinal studies offer a wealth of information, particularly for diseases progression or development studies. In addition, smaller sample sizes are possible to accumulate significant data, as subject assessment is possible at multiple time points.
Animal models are crucial in advancing our knowledge and understanding of pulmonary diseases. Using alternatives models, such as computer simulations, is simply not advanced enough to elucidate all the biological mechanisms inside a living creature.
The latest release of flexiWare (8.2) brings with it the capacity to assess both work of breathing (WOB) and airway compliance, expanding the comprehensive array of pulmonary assessments possible with the flexiVent system.
Asthma is a complex heterogeneous disease whose features have not been completely depicted by any single mouse disease model. In preclinical (in vivo) research, careful considerations should be given to the model employed, its phenotype, as well as the measured outcomes.
Atelectasis or airway closure spontaneously develops following anaesthesia and mechanical ventilation. This progressive loss in lung volume can have a significant impact on the subject’s
The current state-of-the-art in pre-clinical lung function measurements is the evaluation of the mechanical properties of the respiratory system using FOT.
Mice strain can have an important impact on research, as certain strains are better suited to some applications than others.
Discover how to accurately measure lung function in conscious subjects using WBP, HOP, and DCP. Learn why EF50 is a crucial indicator for airway response and respiratory safety pharmacology.
Throughout the global COVID-19 pandemic, there has been a noticeably higher mortality rate in males compared to females. Though the mechanism that causes this remains unknown, Dr. Sharma and Dr. Zeki hypothesize
In May 2021, SCIREQ had the pleasure of hosting a virtual panel discussion with leading preclinical infectious disease researchers focusing on Preclinical Models of COVID-19.
Kumar et al. (2020) recently published a novel manuscript that combined work on cellular and mouse models shows that hyperglycemic conditions and the resultant metabolic alterations cause DNA
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