Evaluating dupilumab and anti-IL-5 in preclinical animal models webinar
September 5, 2023 No Comments
Evaluating dupilumab and anti-IL-5 in preclinical animal models webinar on November 7th at 11am EST
The forced expiration extension (FEV) extension is a hardware add-on that allows the study of expiratory flow limitations, analogous to spirometry, a widely known clinical pulmonary test measuring volumes and flows expired by patients that is used to confirm a diagnostic of respiratory disease or follow treatment. The FEV extension permits the generation of flow-volume loops and its associated parameters by rapidly exposing the subject’s airway opening to negative pressure, referred to as Negative Pressure Forced Expirations (NPFE). The NPFEs can be interspersed with all other flexiVent measurements, uniquely combining the forced expiratory and forced oscillation measurements into a single platform.
NPFE Manoeuvre
During the NPFE manoeuvre, the subject is inflated to total lung capacity (TLC) state and then rapidly switched to a negative pressure reservoir to essentially draw the air out of its lungs. The FEV extension’s outcomes include flow-volume loops, peak expiratory flow (PEF), forced vital capacity (FVC), forced expired volumes (FEVx) and flows (FEFx) at user-defined times are automatically displayed/ calculated by the software.
Available for Mice, rats and ferrets
The FEV extension readily connects to most existing flexiVent systems for use in mice, rats and ferrets. It includes a plethysmograph chamber with an integrated sensor to measure chest flow, a negative pressure reservoir with its controller, and two additional valves. Upon completion of the NPFE, the flexiWare software immediately displays flow-volume loops and automatically calculates all pertinent volume and flow parameters (FEVx, FEFx, FVC, PEF).
Towards Clinical Studies
The addition of FEV offers a means to more directly assess expiratory flow limitation. Although NPFE manoeuvres are obtained under conditions that differ substantially from clinical spirometry, they may provide a useful cross-species correlation for drug development studies. A recent publication by Devos et al, characterized forced expiration measurements in some well-established mouse models of lung diseases, which specific phenotypes were confirmed by a concomitant respiratory mechanics assessment. The researchers observed that disease-induced changes in forced expiration-related charts and parameters were generally similar to what was observed in the clinic. For example, when compared to a control group of healthy mice.
Evaluating dupilumab and anti-IL-5 in preclinical animal models webinar on November 7th at 11am EST
As researchers strive to bridge the gap between preclinical investigations and clinical outcomes, the accurate assessment of Forced Expired Volume (FEV) and Forced Vital Capacity (FVC) emerges as a cornerstone for establishing translational relevance.
This application note highlights the invaluable role of FEV extension’s measurements in facilitating the translation of preclinical findings into tangible benefits for human respiratory health.
Lung function testing is often considered of prime importance in preclinical respiratory research. Being integrative, functional, and translational, the conclusions of such assessments typically impact the decision-making process.
Refined Preclinical Study Design with Spirometry Spirometry is a widely known clinical pulmonary test measuring volumes and flows expired by patients that is used to
Meet the Expert Learn what Dr. Jeroen Vanoirbeek, a scientist at the forefront of the preclinical toxicology research, has to say about his research and
Save time evaluating SCIREQ equipment by having a SCIREQ Application Specialist conduct a custom
literature research. Researchers will receive an email report showing SCIREQ publications relevant to their specific application research area, along with custom equipment recommendations and commentary.
