Evaluating dupilumab and anti-IL-5 in preclinical animal models webinar
Evaluating dupilumab and anti-IL-5 in preclinical animal models webinar on November 7th at 11am EST
Respiratory diseases remain a major global health challenge, creating a continued need for preclinical models that can more accurately reflect clinically relevant disease phenotypes and treatment responses.
As researchers work to strengthen the connection between preclinical and clinical research, Forced Expired Volume (FEV), Forced Vital Capacity (FVC), and other forced-expiration measurements can provide valuable insight into respiratory dysfunction.
The flexiVent® FEV Extension brings these clinically familiar measurements into preclinical research through Negative Pressure Forced Expirations (NPFE) enabling researchers to characterize expiratory flow limitation and generate flow-volume loops alongside comprehensive respiratory mechanics measurements.
One platform. More translational endpoints.
During an NPFE manoeuvre, the subject is carefully inflated to a total lung capacity (TLC) state. The system then rapidly switches to a negative pressure reservoir to essentially draw the air out of the lungs.
Upon completion of the manoeuvre, the flexiWare software automatically displays the flow-volume loops and calculates all critical volume and flow parameters:
PEF: Peak Expiratory Flow
FVC: Forced Vital Capacity
FEVx: Forced Expired Volumes at user-defined times
FEFx: Forced Expired Flows at user-defined times
The addition of the FEV extension offers a reliable means to more directly assess expiratory flow limitation. Although NPFE manoeuvres are obtained under different conditions than clinical spirometry, they provide a highly useful cross-species correlation for drug development and disease modeling.
The FEV extension readily connects to most existing flexiVent systems for use in mice, rats and ferrets. It includes a plethysmograph chamber with an integrated sensor to measure chest flow, a negative pressure reservoir with its controller, and two additional valves. Upon completion of the NPFE, the flexiWare software immediately displays flow-volume loops and automatically calculates all pertinent volume and flow parameters (FEVx, FEFx, FVC, PEF).
The FEV extension is available for use in mice, rats, and ferrets and readily connects to most existing flexiVent systems. The complete add-on hardware suite includes:
A specialized plethysmograph chamber with an integrated sensor to measure chest flow.
A negative pressure reservoir with its own dedicated controller.
Two additional precision valves for rapid pressure switching.
Evaluating dupilumab and anti-IL-5 in preclinical animal models webinar on November 7th at 11am EST
As researchers strive to bridge the gap between preclinical investigations and clinical outcomes, the accurate assessment of Forced Expired Volume (FEV) and Forced Vital Capacity (FVC) emerges as a cornerstone for establishing translational relevance.
This application note highlights the invaluable role of FEV extension’s measurements in facilitating the translation of preclinical findings into tangible benefits for human respiratory health.
Lung function testing is often considered of prime importance in preclinical respiratory research. Being integrative, functional, and translational, the conclusions of such assessments typically impact the decision-making process.
Spirometry is a widely known clinical pulmonary test measuring volumes and flows expired by patients that is used to confirm a diagnostic of respiratory disease or follow treatment. SCIREQ’s flexiVent’s offers an analogous test for preclinical research with its Negative Pressure Forced Expiration (NPFE) extension.
Dr. Jeroen Vanoirbeek is a research Associate Professor at KU Leuven. His interests are occupational and environmental exposure assessment and toxicology, with a special focus on experimental lung immune toxicology.
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