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Integrating Advanced Preclinical Models of Increasing Complexity to Study Lung Injury and Repair

Tuesday, November 10th, at 10am EST / 4:00 PM CET via ZOOM.

SCIREQ & 3RsC will be hosting a free webinar on on Tuesday, November 10th, at 10am EST / 4:00 PM CET via ZOOM.

Title: Integrating Advanced Preclinical Models of Increasing Complexity to Study Lung Injury and Repair

Event Description: This webinar will explore the integration of translational models to study lung injury and repair, specifically focusing on mechanisms in chronic obstructive pulmonary disease, emphysema, and pulmonary hypertension. The discussion will highlight the cross-model application of specialized preclinical methodologies, bridging in vivo assessments like flexiVent invasive lung mechanics testing and micro-computed tomography with ex vivo and in vitro approaches such as precision-cut lung slices and air-liquid interface cultures.

 

Register below to attend:

Speaker Spotlight

Dr. Stefan Hadzic – Justus Liebig University Giessen

Dr. Hadzic is an early career group leader and basic science researcher at the Excellence Cluster Cardio-Pulmonary Institute at Justus Liebig University Giessen. He completed his undergraduate and master’s studies at the University of Belgrade, followed by doctoral research at Justus Liebig University Giessen. His research investigates the molecular mechanisms of alveolar and vascular lung repair in chronic lung diseases, utilizing advanced preclinical methodologies.

LinkedIn: https://www.linkedin.com/in/stefan-hadzic-cpi/

in vivo Respiratory Mechanics Measurements

The flexiVent lung function solution is widely regarded as the gold standard for in vivo respiratory mechanics measurements. It goes beyond traditional resistance and compliance mechanics of pulmonary ventilation, and captures crucial details about the mechanical properties of conducting airways, terminal airways and parenchyma.

The flexiVent achieves the highest sensitivity and reproducibility by precisely controlling experimental conditions.

New Research Platform for Precision Cut Lung Slices

Precision-Cut Lung Slices (PCLS) help researchers understand pathophysiological mechanisms associated with various respiratory diseases. The physioLens is a scientific platform that provides accurate physiological and image-based outcomes in a reproducible fashion. It includes an automated microscope and fluid handling system.

The physioLens features:

  • Automation: The physioLens is capable of capturing images anywhere on slices in a 6 well plate and apply doses without user intervention. Images are analyzed in real-time to provide bronchoconstriction data. 
  • Dosing: Agonists are provided to the slice without user intervention by a dosing nozzle, that can provide a 95% media exchange in less than 20 seconds. In addition, eight dosing bottles are provided to complete a detailed dose response automatically.
  • Image analysis: Images of airways are automatically processed to capture the lumen contours and then calculate bronchoconstriction. 
  • Slice Scanning: Navigating a slice is not necessary with our airway configuration wizard complete with full slice mapping. Fine tune the positions of interest with our click-and-drag XY navigation.

In Vivo and In Vitro Smoke Exposure

We are proud to introduce our new expoCube, adding robust in vitro exposure to the automated inExpose system.

The expoCube provides a novel ALI (air liquid interface) culture exposure system, integrating with the inExpose’s existing exposure generators such as the integrated cigarette smoking robot and industry leading e-cigarette extensions.

The expoCube features:

  • Reproducibility: Efficient, uniform, and precise exposure of particulates onto target cells and tissues.
  • Precision: Optimized flow paths using advanced computational fluid dynamics (CFD) modelling.
  • Deposition: Patented thermophoresis, increasing the deposition efficiency of small particles without imparting unnatural electrostatic charges onto the aerosols.

Request a Free Custom Literature Search

Save time evaluating SCIREQ equipment by having a SCIREQ Application Specialist conduct a custom
literature research. Researchers will receive an email report showing SCIREQ publications relevant to their specific application research area, along with custom equipment recommendations and commentary.