Techniques for imaging the lung have been successfully implemented and validated in humans. These techniques are also of great interest in a preclinical setting, as they provide valuable information on the lung structure and function in a non-invasive manner. This qualitative and quantitative assessment of the lung can therefore be performed in a repetitive manner in order to establish a diagnosis, follow lung growth or disease progression over time, quantify the amount of change or assess the impact of a given therapeutic intervention.
ELIMINATING MOTION ARTIFACTS
At the preclinical level, the acquisition of high resolution images is technically more challenging due to the small size of the structures and the constant motion of the respiratory system which produces artifacts. The programmable aspect of the flexiVent allows advanced gating techniques by enabling image capture during breath hold, which greatly increases image contrast and resolution. Other advantages of this gating technique include the ability to perform respiratory mechanics measurements during the imaging session to complement the study or help image interpretation.
The flexiVent gating protocols could equally be applied to capture high resolution images of other thoracic structures, including cardiac structures, which can also be affected by the motion artifacts of the respiratory system.
- SCIREQ Application Note: Gated Thoracic Imaging with the flexiVent
- Autofluorescence multiphoton microscopy for visualization of tissue morphology and cellular dynamics in murine and human airways. – Kretschmer et al. Lab Invest. 2016 Jul 11. doi: 10.1038/labinvest.2016.69.
- In vivo micro-CT lung imaging via a computer-controlled intermittent iso-pressure breath hold (IIBH) technique – Namati et al. Phys. Med. Biol. 51: 6061, 2006
- Longitudinal assessment of lung cancer progression in the mouse using in vivo micro-CT imaging – Namati et al. Med. Phys. 37: 4793, 2010
- Animals In Synchrotrons: Overcoming Challenges For High‐Resolution, Live, Small‐Animal Imaging – Donnelley et al. AIP Conf Proc 1266: 30, 2010
- In-Vivo Hyperpolarized 3He Lung Imaging in Mice Using x-Centric fGRE Sequence and Custom-Designed flexiVent Ventilator – Ouriadov et al. Proc Intl Soc Mag Reson Med 16, 2008
- Synchrotron phase-constrast X-ray imaging reveals fluid dosing dynamics for gene transfer into mouse airways. – Donnelley et al. Gene Therapy 19: 8, 2012