Developing Inhaled Cannabis Product Methods for in vitro Toxicological Assessment

The current study presented at the 2023 Society of Toxicology aims to assess the toxicological effects of inhaled cannabis products on lung health.

The researchers compare traditional submerged culture methods with air-liquid interface (ALI) cultures, which more closely mimic the lungs’ environment.
They use cannabis vapor extract (CaVE) to capture the cannabinoid profiles of cannabis vapor and an advanced toxicant exposure system called ExpoCube to deliver cannabis vapor to the ALI cultures.
The results indicate that cannabis vapor induces cytotoxicity and a proinflammatory response in lung epithelial cells.
This groundbreaking study provides valuable insights into the potential adverse effects of inhaled cannabis products on lung structural cells.

In Vivo and In Vitro Smoke Exposure

We are proud to introduce the commercialized expoCube, adding robust in vitro exposure to the automated inExpose system.

The expoCube provides a novel ALI (air liquid interface) culture exposure system, integrating with the inExpose’s existing exposure generators such as the integrated cigarette smoking robot and industry leading e-cigarette extensions.

The expoCube features:

Reproducibility: Efficient, uniform, and precise exposure of particulates onto target cells and tissues.
Precision: Optimized flow paths using advanced computational fluid dynamics (CFD) modelling.
Deposition: Patented thermophoresis, increasing the deposition efficiency of small particles without imparting unnatural electrostatic charges onto the aerosols.