This page lists techniques and measurements employed by the physioLens system
The physioLens is equipped with one brightfield LED and has the option of having three fluorescence LEDs. The LED used to excite the channel can be selected instantaneously.
Repeated imaging of multiple airways is accomplished by automated XY stage with micron accuracy thanks to feedback-controlled positioning. A low magnification objective (4x) allows for scanning six entire slices in under 10 minutes. The compiled images allow scientists to find airways in an intuitive manner.
The physioLens currently provides the lumen area of tissue slices, which in turn is used to calculate the bronchoconstriction of the airways of sizes ranging from 50 to over 500 microns. Automated lumen area measurements are accomplished by edge detection algorithms.
Media exchange is accomplished by a nozzle consisting of both an in and an out port. This nozzle enables a media exchange of at least 95% in under 20 seconds.20
