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Exploring ALI Exposures: From Cell Lines to Endpoints

Air Liquid Interface

Traditional submerged cell culture techniques have well-known limitations for aerosol exposure studies; for instance, the impact of exposure dilution within the media and the poor solubility/sedimentation of some exposure particles1. Air liquid interface (ALI) cultures are a technique whereby cells are grown on a microporous membrane insert, immersed in a media-containing well.

Pulmonary Vascular Remodeling

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disorder of the lungs that has been a major public health problem, and has been the third leading cause of death worldwide. A large proportion of COPD deaths (~75%) are linked to tobacco smoking, which is now the primary risk factor in the development of the disease.

Reversal of Emphysema by Restoration of Pulmonary Endothelial Cells

Chronic obstructive pulmonary disease (COPD) is a heterogeneous lung disease that is associated with exposure to inhaled irritants such as cigarette smoke and environmental pollutants. COPD is marked by airway inflammation and emphysema, leading to airflow obstruction and respiratory failure. The pathway to the development of COPD is yet to be fully understood but long-term inflammation is thought to be one of the main drivers.

Animal Models for Pulmonary Diseases

Animal models are crucial in advancing our knowledge and understanding of pulmonary diseases. Using alternatives models, such as computer simulations, is simply not advanced enough to elucidate all the biological mechanisms inside a living creature.