It is essential to trust your research data but it is even more important to validate it. The flexiWare Software is there to help you with that! It manages the entire experimental session and contains validators that perform an initial review. This validated data ensures your outcomes are correct even before attaching the subject to the system.
What are validators?
Validators are key variables associated with various perturbations used to verify that the flexiVent is functioning properly.
Why is data validation so important?
Addressing values outside our recommended ranges early-on, will avoid costly loss of data and time. In many cases you can resolve these issues very quickly and easy.
When should I validate the data?
Large deviations from previous results or recommendations can affect collected data. Therefore it is important to review values prior to subject integration.
Where can I find the validators in the software?
When starting an experimental session, calibrations are performed, summarized and displayed on the calibration wizard or the event log. You should compare these results not only to our recommended calibration values but also to previous study outcomes.
Two of the most important validators shown during the calibration are:
- Deep Inflation (closed) Shunt Resistance (Rl)
- Rl is typically indicative of whether a leak is present in the system or if the system is open to the ambient air.
- For example, in an M2 module we recommend a Rl value above 1125 cmH2O.s/ml. If the values are lower, then you should visually inspect the flexiVent for any faults. It might be necessary to replace common wearable parts such O-rings and tubing. Additionally to that you could apply grease to help the seal, and ensure the Aeroneb is properly attached to the adaptor with solution added.
- SnapShot (open) Tube Resistance (Rt)
- Rt is an indicator of the flexiVent pathway resistance leading to the subject, including the intubation tube or cannula. It is necessary that this pathway has a low enough resistance to allow unimpeded access to the subject’s lungs.
- For example, in an M2 module used with mice, we recommend a Rt value below 0.5 cmH2O.s/ml. In cases where the resistance value is higher, the cannula impedes the signals before reaching the subject consequently impacting the collected physiological data. We recommend a short, thin-wall cannula / intubation tube to reduce any resistive impact (18G for mice, 14G for rats).
For additional information about validators or other data validation techniques, please review the TechNote 007- A Guide to flexiVent Data Validation with flexiWare. This document and others may be found in our Knowledge Center on the SCIREQ website.