Asthma & Airway Hyperresponsiveness


Asthma is a complex disease, whose principal characteristics include reversible airflow obstruction, airway hyperresponsiveness, airway inflammation, mucus hypersecretion and airway remodeling. The disease has reached epidemic proportions worldwide and typically starts early on in life. Asthma can be both chronic and heterogeneous and is often associated with allergies to airborne antigens.

flexiVent


ACCURATE CHALLENGES. DETAILED MEASUREMENTS.
 

Assessing airway responsiveness in response to aerosol challenges is the most frequently reported outcome for asthma research. When equipped with an integrated nebulizer, the flexiVent can be used to both deliver aerosol challenges to a subject’s lungs and follow the developing bronchoconstriction through automated data collection. Using proprietary algorithms, the system can calculate and display an estimate of the dose delivered to the subject’s airway opening. Detailed dose-response curves demonstrating airway hyperresponsiveness are computed and graphed in real-time.

Partitioning the response to describe the contribution from the central airways and tissue mechanics offers additional insight into the disease. It allows researchers to study pathophysiological mechanisms related to airway hyperresponsiveness in mice.

inExpose


standardized processes.
 

Disease models are often generated by exposing subjects to aerosols of allergens using acute or chronic protocols in order to establish relevant asthma phenotypes. The inExpose has been specifically designed to allow for repetitive precise dosage concentration delivery by controlling air flow rates and aerosol generation devices. This is done through automated exposure profiles, which also help to reduce user error and minimize outcome variations among subjects, study, and research groups. The inExpose operates under various configurations and protocols to ensure that the subjects receive repeated yet consistent exposure environments throughout experimentation.

PlethysmographS


compromise between accuracy and invasiveness.
 

In respiratory research, the most specific and precise measurements come at the cost of increased invasiveness. This has been described as the phenotyping uncertainty principle (PUP). For example, while the forced oscillation technique employed by the flexiVent system scores the highest in terms of measurement, accuracy, and precision, it lies the furthest from the subject’s natural state on an invasiveness continuum. On the other hand, as subjects are closer and closer to their natural state when using various plethysmography techniques, measurement specificity and precision register lower and lower on an accuracy continuum. 

Various plethysmography techniques exist to measure lung function in preclinical studies: unrestrained whole body plethysmography (WBP), double chamber plethysmography (DCP), or head-out plethysmography (HOP). While each lung function measurement technique has value, the choice of the technique to be used should be weighed against the research objective and be based on a clear understanding of the technique, the outcomes, and the related liabilities. Measurements using plethysmography systems are done in conscious subjects where various external factors significantly impact the outcomes obtained. 

tremoFlo


translational non-invasive technique.
 

High frequency airwave oscillometry assesses changes in resistance and reactance non-invasively in conscious large animals, such as dogs or non-human primates. These measurements are obtained simultaneously to conventional ventilatory parameters such as respiratory rate, tidal volume, or minute ventilation. The technique has translational value since it is also used in the clinic to assess patients.

Tissue baths


cLASSiCal pharmacology.
 

The airway smooth muscle plays an undeniable role in asthma. The excessive amount of contraction it can generate as well as the enhanced sensitivity have been associated with key characteristics of asthma, namely bronchoconstriction and airway hyperresponsiveness. Studying its intrinsic ability to contact or relax in an isolated tissue bath where external influences can be removed can offer complimentary data to in vivo lung function measurements. This technique is a classic pharmacological tool that can be applied to various types of contractile tissues from different species. 

Asthma